Coding

Part:BBa_K3513002:Design

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


endoglucanase with signal peptide, linker and promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 147
    Illegal NotI site found at 1291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1204
    Illegal BamHI site found at 340
    Illegal XhoI site found at 702
    Illegal XhoI site found at 951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 102
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 1407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 386


Design Notes

Bind a engineered endoglucanase with a promoter


Source

Origin source of endoglucanase: Streptomyces Coelicolor. But the team engineered it.

References